Part:BBa_K1973016
pleD*
pleD* BBa_K1973016 Codifies the PleD* protein from Caulobacter crescentus, which is a diguanilate cyclase that induces the biofilm formation by increasing c-di-GMP (cyclic diguanilate monophosphate) levels. c-diGMP regulates both the formation and dispersion of the biofilm, depending on the quantity of it that is present inside the cell. High levels of c-diGMP will increase the biofilm formation, and low levels of it will induce its dispersion. To manipulate this, we will make use of a phosphodiesterase, that will destroy the c-diGMP, and a diguanilate cyclase, that will produce it, YhjH and PleD* respectively. Contains a constitutive mutation in the original sequence (see BBa_K2012002) that improves the enzymatic activity by knocking out the regulation system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 29
Illegal BamHI site found at 1186 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 444
Illegal NgoMIV site found at 591
Illegal AgeI site found at 927 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 102
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